Investigations were made to explore the influence of lowering twist level on quality characteristics of rotor spun yarn. Three levels of yarn linear density (i.e. 40, 35 and 30 tex) and five levels of twist (i.e. 700, 600, 550, 500, and 450) were employed during yarn spinning trials. Each twist multiple was investigated at all linear densities for tensile strength, elongation, total CVm (Coefficient of Mass Variation) imperfection index and hairiness. 100% cotton yarn samples were prepared on Reiter R-40 at rotor speed of 90,000 rpm. Determination of elongation, yarn strength, hairiness, mass variation, and total imperfections index was carried out on Uster Tensorapid-4 and Uster Tester-4 as per set standards of ISO standard test methods. Based on investigations it is established that yarn strength and elongation declined minutely (Insignificant) with lowering twist levels but still can be confidently used for knitting yarns. However, significant improvement in total imperfection index and marginal enhancement in CVm were experienced.